IFCA –
Immunofluorescent Comet Assay
We
have developed a novel staining approach of the comet
assay (CA),
which enables us to visualize even a single chromatin fibers and thus
allows us
to take a closer look at the origin of the comet
tail. This new method is based on the immunofluorescence detection of
the chromatin
core proteins, which are still bound to DNA after the neutral
electrophoresis.
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 |
 |
 |
| Neutral
CA: staining with SybrGreen |
Neutral
IFCA: immunofluorescent staining of the chromatin core proteins, which are still
bound to DNA |
2D
Neutral IFCA: 10 min electrophoresis in one direction and 10 min
electrophoresis into perpendicular direction |
Alkaline
IFCA: despite denaturing lysis and unwinding conditions
chromatin core
proteins still can be detected |
| DNA
Damage and Repair Topics |
|||
| Laser microbeam induced DNA damages and repair | |||
| Former
DNA
Damage and Repair Topics |
|||
| UV-A
Induced DNA Damage |
DNA Double Strand Repair | Comet-assay
and Comet-FISH |
Intrinsic Oxidative
DNA Damages |