Head


Jan Peter Tuckermann
Jan Peter Tuckermann

Postdocs

Sabine Connert

Anna Kleyman

Kerstin Minnich

PhD Students

Ulrike Baschant

Sabine Hübner

Anita Neumann

Alexander Rauch

Technicians

Susanne Ostermay

Guests

Stefanie Czieso (apprentice)


Former Lab Members

Anett Illing



Tuckermann Laboratory

Tissue-specific hormone action

Tissue specific action of glucocorticoid hormone receptors and interacting factors in age related processes: stem cell renewal / differentiation, inflammation, osteoporosis

Steroid hormones exert a wide range of physiological effects in virtual all tissues of the body. In addition they are used as therapeutic compounds as for instance glucocorticoids. These hormones exert a wide range of anti-inflammatory and immunosuppressive activities after binding to the glucocorticoid receptor. Their wide spread medical use for the treatment of inflammatory disorders is hampered by severe side effects. Therefore a precise understanding of the molecular and cellular mechanism is essential in order to improve anti-inflammatory and anti-cancer therapies.

 

Projects

Our research group is interested in the impact of the glucocorticoid receptor and estrogen receptors and interacting transcription factors such as AP-1, NFκB and STAT5 in the immune system (Cell. 93, 531-541; J Cell Biol. 147, 1365-1370; EMBO J. 20, 7168-7173), bone metabolism (osteoporosis) and the self-renewal / differentiation of haematopoietic stem cells and the development of cancer. The approach includes the analysis of cell type and function selective knock out mice in a variety of disease models in combination with the characterization of cell biological functions of steroid receptors in primary cells of these mice.

 

» X-Gal staining of a cbfa1cre transgenic mouse crossed with Rosa26 exhibiting recombination in osteoblasts. Those mice allow to inactivate the genes for the Glucocorticoid receptor and other genes in osteoblasts in order to measure the contribution of those factors in glucocorticoid induced osteoporosis.

 

 

» Langerhans Cell staining with MHCII antibodies in the mouse epdidermis, initiating or modulating immune responses affected by glucocorticoids.

 

 

» Raster electron micrograph of an activated bone marrow derived macrophage.

 

 

 

Recent selected publications

  • Maes C*, Goossens S*, Bartunkova S*, Drogat B, Coenegrachts L, Stockmans I, Moersmans K, Nyabi O, Haigh K, Naessens M, Haenebalcke L, Tuckermann JP, Tjwa M, Carmeliet P, Mandic V, David JP, Behrens A, Nagy A, Carmeliet G*, Haigh JJ* (2009) Increased skeletal VEGF enhances b-catenin activity and results in excessively ossified bones. EMBO Journal [epub ahead of print]
    *Equal contribution [PubMed]
  • Parlato R, Otto C, Tuckermann J, Stotz S, Kaden S, Grone HJ, Unsicker K, Schutz, G. (2009) Conditional inactivation of glucocorticoid receptor gene in dopamine-b-hydroxylase cells impairs chromaffin cell survival. Endocrinology . 150,1775-17781. [PubMed]
  • Preusch R, Rattazi M, Albrecht C, Merle U, Tuckermann J, Schütz G, Blessing E, Zoppellaro G, Pauletto, P, Krempien R Rosenfeld ME, Katus HA, Bea F.(2008) Critical Role of Macrophages in Glucocorticoid Driven Vascular Calcification in a Mouse-Model. Arterioscler Thromb Vasc Biol. 28(12):2158-64. [PubMed]
  • Wüst S, van den Brandt J, Tischner D, Kleiman A, Tuckermann J, Gold R, Lühder F, Reichardt HM. (2008) Peripheral but not NCS-residing T cells are the therapeutic targets of glucocorticoids in experimental autoimmune encephalomyelitis. J Immunol. 180, 8434-43. [PubMed]
  • Bayo P, Sanchis A, Bravo A, Cascallana JL, Buder K, Tuckermann J, Schütz G, Pérez P. (2008) Glucocorticoid receptor is required for skin barrier competence. Endocrinology 149:1377-88 [PubMed]
  • Textor B, Licht AH, Tuckermann JP, Jessberger R, Razin E, Angel P, Schorpp-Kistner M, Hartenstein B. (2007) JunB is required for IgE-mediated degranulation and cytokine release of mast cells. J Immunol. 179, 6873. [PubMed]
  • Kleiman A, Tuckermann JP. (2007) Glucocorticoid receptor action in beneficial and side effects of steroid therapy: lessons from conditional knockout mice. Mol Cell Endocrinol. 275, 98-108. [PubMed]
  • Tuckermann JP, Kleiman A, Moriggl R, Spanbroek R, Neumann A, Illing A, Clausen BE, Stride B, Förster I, Habenicht AJ, Reichardt HM, Tronche F, Schmid W, Schütz G. (2007) Macrophages and neutrophils are the targets for immune suppression by glucocorticoids in contact allergy. J Clin Invest. 117, 1381-1390. [PubMed]
  • Tuckermann JP, Kleiman A, McPherson KG, Reichardt HM. (2005) Molecular mechanisms of glucocorticoids in the control of inflammation and lymphocyte apoptosis. Crit Rev Clin Lab Sci. 42, 71-104. [PubMed]
  • Tuckermann JP, Reichardt HM Göttlicher M, Vujic M, Weih F, Angel P, Herrlich P, Schütz G. (2001) Repression of inflammatory responses in the absence of DNA binding by the glucocorticoid receptor. EMBO J . 20, 7168-73. [PubMed]

 

Last update: July 15, 2009
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