fli-leibniz.de
Grosse Laboratory
Biochemistry
Genomic instability and DNA replication
The doubling of genetic information, namely DNA replication, is a central aspect of all living organisms. Errors occurring during DNA replication may lead to cancer or premature aging of the cell and/or the whole organism. The Grosse group is interested in basic aspects of DNA replication, its regulation and the prevention of errors that might occur during this process.
The questions we are addressing are: (i) how is the initiation of replication regulated and how is it stopped when something goes awry; (ii) what happens to errors that are introduced during this process, and (iii) what happens to replication forks that are halted in front of a damaged site; (iv) how are stalled replication forks started again and (v) what are the signals that cause cell death when damaged sites are irreparable.
We intend that our research contributes to a better understanding of how cells prevent replication errors and thereby escape premature senescence and ultimately cell death.
The questions we are addressing are: (i) how is the initiation of replication regulated and how is it stopped when something goes awry; (ii) what happens to errors that are introduced during this process, and (iii) what happens to replication forks that are halted in front of a damaged site; (iv) how are stalled replication forks started again and (v) what are the signals that cause cell death when damaged sites are irreparable.
We intend that our research contributes to a better understanding of how cells prevent replication errors and thereby escape premature senescence and ultimately cell death.
» Model of the eukaryotic DNA replication fork
Projects
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Replication initiation - see here on our group's web site
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A Zebrafish model for studying replication initiation - see here on our group's web site
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DNA helicases - see here on our group's web site
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Proteins that interact with replication factors and with p53 - see here on our group's web site
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Microtubules and Kinesin - see here on our group's web site
Recent selected publications
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Jain A, Bacolla A, Chakraborty P, Grosse F, Vasquez KM (2010) Human DHX9 helicase unwinds triple-helical DNA structures. Biochemistry, 49, 6992-6999. [PubMed]
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Chakraborty P, Grosse F (2010) WRN helicase unwinds Okazaki fragment-like hybrids in a reaction stimulated by the human DHX9 helicase. Nucleic Acids Res, 38, 4722-4730. [PubMed]
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Baum N, Schiene-Fischer C, Frost M, Schumann M, Sabapathy K, Ohlenschläger O, Grosse F, Schlott B (2009) The prolyl cis/trans isomerase cyclophilin 18 interacts with the tumor suppressor p53 and modifies its functions in cell cycle regulation and apoptosis. Oncogene, 28, 3915-3925. [PubMed]
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Bauerschmidt C, Pollok S, Kremmer E, Nasheuer HP, Grosse F (2007) Interactions of human Cdc45 with the Mcm2-7 complex, the GINS complex, and DNA polymerases delta and epsilon during S phase. Genes Cells, 12, 745-758. [PubMed]
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Rockstroh A, Kleinert A, Kramer M, Grosse F, Søe K (2007) Cellular stress triggers the human topoisomerase I damage response independently of DNA damage in a p53 controlled manner. Oncogene, 26, 123-131. [PubMed]
Last update: Sep 29, 2011
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